期刊
JOURNAL OF GINSENG RESEARCH
卷 47, 期 2, 页码 237-245出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2022.08.001
关键词
ginsenoside Rg2; atherosclerosis; endothelial cell; inflammation; signaling pathway
Ginsenoside Rg2 inhibits the expression of inflammatory factors in vascular smooth muscle cells and human umbilical vein endothelial cells by blocking the NF-KB and p-ERK signaling pathways. Moreover, Rg2 can also suppress the proliferation, migration, and phenotypic transformation of vascular smooth muscle cells induced by platelet derived growth factor-BB (PDGF-BB), and reduce intimal proliferation and regulate inflammatory pathways in a rat carotid balloon injury model.
Background: Ginsenoside Rg2 (Rg2) has a variety of pharmacological activities and provides benefits during inflammation, cancer, and other diseases. However, there are no reports about the relationship between Rg2 and atherosclerosis.Methods: We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to detect the cell viability of Rg2 in vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). The expression of inflammatory factors in HUVECs and the expression of phenotypic transformation-related marker in VSMCs were detected at mRNA levels. Western blot method was used to detect the expression of inflammation pathways and the expression of phenotypic transformation at the protein levels. The rat carotid balloon injury model was performed to explore the effect of Rg2 on inflammation and phenotypic transformation in vivo. Results: Rg2 decreased the expression of inflammatory factors induced by lipopolysaccharide in HUVECs-without affecting cell viability. These events depend on the blocking regulation of NF-KB and p-ERK signaling pathway. In VSMCs, Rg2 can inhibit the proliferation, migration, and phenotypic transformation of VSMCs induced by platelet derived growth factor-BB (PDGF-BB)-which may contribute to its anti-atherosclerotic role. In rats with carotid balloon injury, Rg2 can reduce intimal proliferation after injury, regulate the inflammatory pathway to reduce inflammatory response, and also suppress the phenotypic transformation of VSMCs.Conclusion: These results suggest that Rg2 can exert its anti-atherosclerotic effect at the cellular level and animal level, which provides a more sufficient basis for ginseng as a functional dietary regulator.(c) 2022 The Korean Society of Ginseng. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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