期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
卷 19, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/ijerph19148672
关键词
COVID-19; SARS-CoV-2; surface disinfection; clinical equipment; office supplies; ozone
资金
- Instituto de Salud Carlos III, Madrid, Spain
- European Regional Development Funds (FEDER) [COV20/00702]
- Lighting Dynamic Technology, S.L., Las Palmas, Spain
- Instituto de Salud Carlos III, Madrid, Spain [COV20/00702]
- Fundacion Canaria del Instituto de Investigacion Sanitaria de Canarias (FIISC), Las Palmas, Spain [PIFIISC20/19]
- Fundacion Mapfre Guanarteme, Las Palmas, Spain [OA20/072]
- Gobierno de Canarias, Las Palmas, Spain [PI2020010357]
This study assessed the optimal conditions of ozone treatment for eliminating heat-inactivated SARS-CoV-2 from office supplies and clinical equipment. Results showed that ozone could effectively eliminate the virus RNA, providing an additional tool to control the spread of the COVID-19 pandemic.
(1) Background: Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) continues to cause profound health, economic, and social problems worldwide. The management and disinfection of materials used daily in health centers and common working environments have prompted concerns about the control of coronavirus disease 2019 (COVID-19) infection risk. Ozone is a powerful oxidizing agent that has been widely used in disinfection processes for decades. The aim of this study was to assess the optimal conditions of ozone treatment for the elimination of heat-inactivated SARS-CoV-2 from office supplies (personal computer monitors, keyboards, and computer mice) and clinical equipment (continuous positive airway pressure tubes and personal protective equipment) that are difficult to clean. (2) Methods: The office supplies and clinical equipment were contaminated in an area of 1 cm(2) with 1 x 10(4) viral units of a heat-inactivated SARS-CoV-2 strain, then treated with ozone using two different ozone devices: a specifically designed ozonation chamber (for low-medium ozone concentrations over large volumes) and a clinical ozone generator (for high ozone concentrations over small volumes). SARS-CoV-2 gene detection was carried out using quantitative real-time polymerase chain reaction (RT-qPCR). (3) Results: At high ozone concentrations over small surfaces, the ozone eliminated SARS-CoV-2 RNA in short time periods-i.e., 10 min (at 4000 ppm) or less. The optimum ozone concentration over large volumes was 90 ppm for 120 min in ambient conditions (24 degrees C and 60-75% relative humidity). (4) Conclusions: This study showed that the appropriate ozone concentration and exposure time eliminated heat-inactivated SARS-CoV-2 RNA from the surfaces of different widely used clinical and office supplies, decreasing their risk of transmission, and improving their reutilization. Ozone may provide an additional tool to control the spread of the COVID-19 pandemic.
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