4.3 Article

A chromosome-level, fully phased genome assembly of the oat crown rust fungus Puccinia coronata f. sp. avenae: a resource to enable comparative genomics in the cereal rusts

期刊

G3-GENES GENOMES GENETICS
卷 12, 期 8, 页码 -

出版社

OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkac149

关键词

oat; rust; virulence; genome; chromosome; isolate Pca203

资金

  1. PepsiCo Inc.
  2. College of Food, Agriculture, and Natural Resource Science at the University of Minnesota
  3. CSIRO Research Office Postdoctoral Fellowship
  4. ARC Discovery Early Career Researcher Award [DE190100066]
  5. USDA-ARS/The University of Minnesota [3002-11031-00053115]
  6. Australian Research Council [DE190100066] Funding Source: Australian Research Council

向作者/读者索取更多资源

Advances in sequencing technologies have allowed the generation of fully phased genome references for organisms with nonhaploid genomes. This study successfully generated a chromosome-scale assembly for the oat crown rust pathogen, which provides valuable resources for studying pathogen effectors and virulence evolution.
Advances in sequencing technologies as well as development of algorithms and workflows have made it possible to generate fully phased genome references for organisms with nonhaploid genomes such as dikaryotic rust fungi. To enable discovery of pathogen effectors and further our understanding of virulence evolution, we generated a chromosome-scale assembly for each of the 2 nuclear genomes of the oat crown rust pathogen, Puccinia coronata f. sp. avenae (Pca). This resource complements 2 previously released partially phased genome references of Pca, which display virulence traits absent in the isolate of historic race 203 (isolate Pca203) which was selected for this genome project. A fully phased, chromosome-level reference for Pca203 was generated using PacBio reads and Hi-C data and a recently developed pipeline named NuclearPhaser for phase assignment of contigs and phase switch correction. With 18 chromosomes in each haplotype and a total size of 208.10 Mbp, Pca203 has the same number of chromosomes as other cereal rust fungi such as Puccinia graminis f. sp. tritici and Puccinia triticina, the causal agents of wheat stem rust and wheat leaf rust, respectively. The Pca203 reference marks the third fully phased chromosome-level assembly of a cereal rust to date. Here, we demonstrate that the chromosomes of these 3 Puccinia species are syntenous and that chromosomal size variations are primarily due to differences in repeat element content.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据