Seasonal variation of immune response to heterologous erythrocytes in natural populations of red-backed (Clethrionomys rutilus) and gray-sided (C. rufocanus) voles in Western Siberia

We studied the seasonal variation of adaptive humoral immunity (AHI) in northern red-backed vole (Clethrionomys rutilus Pallas, 1779, RBV) and gray-sided vole (C. rufocanus Sundevall, 1846, GSV) in Tomsk region of Western Siberia. Immunoresponsiveness (IR) to sheep red blood cells was assessed by the number of antibody-producing cells in the spleen. The use of a generalized linear model to analyze the effects of species, sex, year of research, and season of withdrawal of individuals from nature on IR showed a significant effect of species identity, season of animal capture, and the interaction of species with season. The RBV demonstrated higher immune responses during a year, and both species had higher IR in winter. Suppression of IR in spring was greater, started earlier, and lasted longer (March-May) in GSV. In RBV, immunosuppression was restricted to April. The significant negative within year correlations of IR with body mass and masses of reproductive organs in GSV indicated a trade-off between AHI and growth and reproduction processes. A probable explanation for the difference between species in the seasonal variation of AHI may be related to the difference in tropho-energetic requirements of each vole species. GSV is a predominantly herbivorous rodent and its thermoregulation seems less efficient than of RBV. The deeper spring immunosuppression in GSV may explain in part its higher mortality during the season of colds.

Automated summary

The study revealed differences in the seasonal variation of adaptive humoral immunity between the northern red-backed vole and gray-sided vole in Western Siberia. Both species showed higher immune responses in winter, with spring immunosuppression being more severe in the gray-sided vole. Negative correlations within year of immune responsiveness with body mass and reproductive organ mass indicated a trade-off between AHI and growth and reproduction processes in the gray-sided vole.