4.7 Article

IL-1R/C3aR signaling regulates synaptic pruning in the prefrontal cortex of depression

期刊

CELL AND BIOSCIENCE
卷 12, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s13578-022-00832-4

关键词

Complement C3; C3aR; Synaptic pruning; Palmitoylation; Depression

资金

  1. Education Department of Fujian Province [2019-WJ-38]
  2. Xiamen Municipal Health Commission [2019-WJ-38]
  3. Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program [CXTD22002]
  4. National Natural Science Foundation of China [82060757]

向作者/读者索取更多资源

This study investigates the dynamic alteration of complement C3 and its receptor C3aR during depression and the mechanism of astrocyte-microglia IL-1R/C3/C3aR on synaptic pruning. The activation of complement C3/C3aR may be helpful in predicting the onset stage of depression.
Background Major depressive disorder is characterized by not only monoamine neurotransmitters deficiencies but also persistent neuroinflammation. The complement system is an attractive therapeutic target for various inflammation-related diseases due to its early activation in inflammatory processes. Results In the present study, the dynamic alteration of complement C3 and its receptor C3aR during the occurrence of depression and the mechanism of astrocyte-microglia IL-1R/C3/C3aR on synaptic pruning were investigated. The proteomic analysis firstly showed that chronic stress caused an elevation of C3. GO analysis indicated that complement system-mediated synaptic pruning signaling was involved in depression. The dynamic observation indicated that C3/C3aR was activated in the early onset and throughout the course of depression induced by lipopolysaccharide (LPS) and chronic stress. In contrast, C3aR blockade inhibited the hyperactivation of microglial APT2/DHHC7 palmitoylation cycle, which mediated the translocation of STAT3 and the expression of proinflammatory cytokines. Meanwhile, C3aR blockade also attenuated the synaptic pruning and enhanced the synaptogenesis in the prefrontal cortex of mice. Moreover, the blockade of IL-1R/NF-kappa B signaling pathway reduced the release of C3 from astrocyte. Conclusions The current study demonstrates that astrocyte-microglia IL-1R/C3/C3aR activation causes the abnormal synaptic pruning in depression, and suggests that the activation of complement C3/C3aR may be particularly helpful in predicting the onset stage of depression.

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