期刊
ADVANCED HEALTHCARE MATERIALS
卷 11, 期 16, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202200268
关键词
antifungal; fungal biofilms; fungal resistance; photodynamic therapy; polypeptides
资金
- National Natural Science Foundation of China [51773196, 51573184, 51473029, 51833010]
- Youth Innovation Promotion Association of CAS [2017266]
In this study, a photosensitizer-polypeptide conjugate was synthesized for effective antifungal and antibiofilm treatment. The conjugate showed enhanced binding ability to Candida albicans and fluconazole-resistant Candida albicans through electrostatic interactions. It exhibited effective antifungal efficiency and improved penetration in a Candida albicans biofilm, effectively eliminating the biofilm by photodynamic effects. In a fluconazole-resistant Candida albicans-infected rat model, the conjugate demonstrated significantly enhanced therapeutic effects with minimal side effects.
Persistent fungal infections caused by biofilms seriously endanger human health. In this study, a photosensitizer-polypeptide conjugate (PPa-cP) comprising a photosensitizer, pyropheophorbide a (PPa), and a cationic polypeptide (cP) is readily synthesized for effective antifungal and antibiofilm treatment. Compared with free PPa, the cationic PPa-cP shows enhanced binding ability to the negatively charged surface of Candida albicans (C. albicans) through electrostatic interactions. As a result, PPa-cP exhibits effective antifungal efficiency against both C. albicans and fluconazole-resistant C. albicans in vitro under light irradiation. The minimum inhibitory concentration (MIC) of PPa-cP for both C. albicans and fluconazole-resistant C. albicans is 1 mu m. In addition, PPa-cP also shows improved penetration in a C. albicans biofilm, thus effectively eliminating the C. albicans biofilm by photodynamic effects. More importantly, PPa-cP demonstrats significantly enhanced therapeutic effects in a fluconazole-resistant C. albicans-infected rat model with minimal side effects. In conclusion, the current work presents an effective strategy to combat biofilm infections associated with biomedical equipment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据