Elevated temperature and carbon dioxide levels alter growth rates and shell composition in the fluted giant clam, Tridacna squamosa

Giant clams produce massive calcified shells with important biological (e.g., defensive) and ecological (e.g., habitat-forming) properties. Whereas elevated seawater temperature is known to alter giant clam shell structure, no study has examined the effects of a simultaneous increase in seawater temperature and partial pressure of carbon dioxide (pCO(2)) on shell mineralogical composition in these species. We investigated the effects of 60-days exposure to end-of-the-century projections for seawater temperature (+ 3 & DEG;C) and pCO(2) (+ 500 & mu;atm) on growth, mineralogy, and organic content of shells and scutes in juvenile Tridacna squamosa giant clams. Elevated temperature had no effect on growth rates or organic content, but did increase shell [Mg-24]/[Ca-40] as well as [Ca-40] in newly-formed scutes. Elevated pCO(2) increased shell growth and whole animal mass gain. In addition, we report the first evidence of an effect of elevated pCO(2) on element/Ca ratios in giant clam shells, with significantly increased [Ba-137]/[Ca-40] in newly-formed shells. Simultaneous exposure to both drivers greatly increased inter-individual variation in mineral concentrations and resulted in reduced shell N-content which may signal the onset of physiological stress. Overall, our results indicate a greater influence of pCO(2) on shell mineralogy in giant clams than previously recognized.

Automated summary

This study investigated the effects of seawater temperature and carbon dioxide partial pressure on the mineral composition of giant clam shells. The results showed that elevated temperature had no effect on growth and organic content, but increased the ratio of magnesium to calcium in the shell. Elevated carbon dioxide partial pressure increased shell growth and overall body mass gain. Additionally, it was found that elevated carbon dioxide partial pressure had an effect on the element/calcium ratio in giant clam shells. Simultaneous exposure to both factors increased inter-individual variation in mineral concentrations and resulted in reduced nitrogen content in the shell.