Anti-Biofilm Activities of Chinese Poplar Propolis Essential Oil against Streptococcus mutans

Streptococcus mutans (S. mutans) is a common cariogenic bacterium that secretes glucosyltransferases (GTFs) to synthesize extracellular polysaccharides (EPSs) and plays an important role in plaque formation. Propolis essential oil (PEO) is one of the main components of propolis, and its antibacterial activity has been proven. However, little is known about the potential effects of PEO against S. mutans. We found that PEO has antibacterial effects against S. mutans by decreasing bacterial viability within the biofilm, as demonstrated by the XTT assay, live/dead staining assay, LDH activity assay, and leakage of calcium ions. Furthermore, PEO also suppresses the total of biofilm biomasses and damages the biofilm structure. The underlying mechanisms involved may be related to inhibiting bacterial adhesion and GTFs activity, resulting in decreased production of EPSs. In addition, a CCK8 assay suggests that PEO has no cytotoxicity on normal oral epithelial cells. Overall, PEO has great potential for preventing and treating oral bacterial infections caused by S. mutans.

Automated summary

This study found that propolis essential oil (PEO) has antibacterial effects on cariogenic bacterium Streptococcus mutans (S. mutans) by decreasing bacterial viability within the biofilm and damaging the biofilm structure. The underlying mechanisms may involve inhibiting bacterial adhesion and glucosyltransferases (GTFs) activity, resulting in decreased production of extracellular polysaccharides (EPSs). Additionally, PEO showed no cytotoxicity on normal oral epithelial cells.