期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 776, 期 -, 页码 18-24出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2016.06.033
关键词
Cholesterol oxidase; Cholesterol; Amperometric biosensor; Boronic acid; Reconstitution; Electropolymerization
资金
- National Boron Research Institute (BOREN) of Turkey [2011-C0296]
- Scientific and Technological Research Council of Turkey (TUBITAK) [110T508]
An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinudeotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiephene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic add. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed. (C) 2016 Elsevier B.V. All rights reserved.
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