期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 761, 期 -, 页码 112-117出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2015.12.014
关键词
Graphene; Chitosan-ferrocene; Fe3O4/Au NPs; Signal amplification; Carcinoembryonic antigen
资金
- National Natural Science Foundation of China [21265012, 21265017, 21475056]
- Program for New Century Excellent Talents in University [NCET-13-0848]
A novel electrochemical immunosensor based on a signal amplification strategy was developed for sensitive detection of carcinoembryonic antigen (CEA). Graphene/chitosan-ferrocene (GO/CS-Fc) was employed as the matrix to immobilize the primary antibodies (Ab1). Owing to the larger surface area, a large amount of Ab1 was captured on GO/CS-Fc sensor platform, thus amplifying the detection signal. Fe3O4/Au NPs were functionalized as the label to conjugate with the secondary antibodies (Ab2). Due to the homogeneous and narrow size distribution of Fe3O4/Au NPs, several Ab2 were bound on each nanoparticle, thus enhanced sensitivity was achieved by introducing the Fe3O4/Au NPs/Ab2 onto the electrode surface through sandwich immunoreactions. On the basis of p-aminophenol (AP) redox cycling amplification strategy, the developed immunosensor showed a 10-fold increase in detection signal compared to the immunosensor without Fe3O4/Au NPs labeling. The proposed immunosensor offered a sensitive determination of CEA in the range of 0.001-30 ng mL(-1) with a detection limit of 0.39 pg/mL. This immunoassay method would open up a promising platform to detect various tumor markers for diagnosis of different cancers. (C) 2015 Elsevier B.V. All rights reserved.
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