期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 780, 期 -, 页码 303-310出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2016.09.028
关键词
Sthiol-containing compounds; L-Cysteine; Cobalt phthalocyanine; Screen-printed electrodes; Embryo cell culture media; Oxidative stress
资金
- programme Salvador de Madariaga from the Ministerio de Economia y Competitividad [PRX14/00363]
- MICINN-FEDER (Spain) [CTQ2013-48280-C3-3-R]
Thiol-containing compounds such as L-cysteine have been demonstrated to play an important role in metabolism and cellular growth, acting as powerful antioxidants. Consequently, their analytical determination in biological media has received a considerable amount of attention. In this work, an electrochemical sensor for the accurate electroanalytical determination of L-cysteine is proposed, based upon a Co(II)-phthalocyanine bulk modified disposable screen-printed graphite electrode (CoPc-SPE). Cyclic (CV) and Square Wave (SWV) voltammetry experiments have demonstrated an excellent electrocatalytic activity towards the electrochemical oxidation of Lcysteine using CoPc-SPEs within optimum neutral or basic pH. Moreover, the SWV response of L-cysteine is found to exhibit a linear range of 2.6-200 mu M, with a low limit of detection of 4 mu M (S/N = 3) and a sensitivity of 0.78 mu A cm(-2) mu M-1. Coefficient of variation for reproducibility and repeatability of L-cysteine determination using the CoPc-SPE are 3 and 0.4%, respectively. The effect of inherent interferences such as naturally occurring amino acids, cystine and cysteic acid has been also evaluated.. Finally, the applicability of the L-cysteine electrochemical sensor based upon CoPc-SPEs has been successfully demonstrated for the first time for the assessment of L-cysteine in a complex embryo cell culture medium. Crown Copyright (C) 2016 Published by Elsevier B.V. All rights reserved.
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