期刊
DRUG DESIGN DEVELOPMENT AND THERAPY
卷 16, 期 -, 页码 2545-2557出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/DDDT.S357891
关键词
CML; flavagline; cycle arrest; apoptosis; PI3K; Akt; mTOR; JAK2; STAT3; MAPK
资金
- National Natural Science Foundation of China [81872772, U 1812403, 81960546, 81760630, 82160808, 82160813, 81871313]
- Guizhou Provincial Natural Science Foundation [QKHPTRC [2020] 5008, QKHZC [2020] 4Y203, QKHJC-ZK [2022] YB293, QKHPTRC [2019] 5627, QKHJC-ZK [2021] YB569, QKHZC [2019] 2762, QKHJC-ZK [2022] YB297, QKHJC [2020] 1Y033, [2016] 4002, [2019] 5406]
- Foundation of State Key Laboratory of Functions and Applications of Medicinal Plants [FAMP201901K, QZYY-019-022]
- State Key Laboratory of Drug Research [SIMM2105KF-15]
MQ-16 shows significant inhibitory effects on the proliferation of K562 cells and induces cell cycle arrest at the G2/M phase in a time- and concentration-dependent manner. It induces mitochondria-dependent apoptosis by downregulating anti-apoptotic proteins Bcl-2 and Bcl-xL, and causes time- and concentration-dependent DNA fragmentation. Furthermore, MQ-16 affects the expression of PI3K/Akt/mTOR, JAK2/STAT3, and MAPK pathway-related proteins.
Purpose: Chronic myelogenous leukemia (CML) is a hematological malignancy with increased proliferation of cells of the myeloid series. This can disrupt normal hematopoiesis. The 1-(2-(dimethylamino)acetyl)-rocaglaol (MQ-16) is a new synthetic flavagline compound that showed promising activity in chronic myeloid leukemia K562 cells. This study aims to analyze the underlying mechanisms of MQ-16 against CML.Methods: Growth, cell cycle progression, and apoptosis were assessed in K562 cells following MQ-16 exposure by MTT assay and flow cytometry. The effect of MQ-16 on DNA strands between nucleosomes was examined by 1% agarose gel electrophoresis. PI3K/ Akt/mTOR, JAK2/STAT3, and mitogen-activated protein kinase (MAPK) pathway-related proteins were detected in MQ-16-treated K562 cells by Western blot.Results: MQ-16 significantly inhibited the proliferation of K562 cells and arrested the cell cycle at the G2/M phase in a time-and concentration-dependent manner. MQ-16 induced mitochondria-dependent apoptosis by downregulating the anti-apoptotic proteins Bcl-2 and Bcl-xL and induced time-and concentration-dependent DNA fragmentation. In addition, MQ-16 affected the expression of PI3K/Akt/mTOR, JAK2/STAT3, and MAPK pathway-related proteins.Conclusion: In summary, MQ-16 appears to be a promising chemotherapeutic drug for treating CML.
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