期刊
CELL ADHESION & MIGRATION
卷 16, 期 1, 页码 94-106出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19336918.2022.2103925
关键词
Fluid shear stress; autophagy; LCSCs; migration
类别
资金
- China Postdoctoral Science Foundation [2020M672265]
- Key scientific and technological project of Henan Province [212102310608]
- Joint Construction Project of Henan Province Medical Science and Technology Key Research Plan [LHGJ20200274]
Fluid shear stress promotes the migration of liver cancer stem cells via the RhoA-YAP1-autophagy pathway.
Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC), but the role of the RhoA-YAP1-autophagy pathway in HCC remains unclear. Due to the core role of liver cancer stem cells (LCSCs) in HCC metastasis and recurrence, we explored the RhoA-YAP1-autophagy pathway in LCSCs under FSS. Our results indicate that LCSCs have stronger proliferation and cell spheroidization abilities. FSS (1 dyn/cm(2)) upregulated the migration of LCSCs and autophagy protein markers, inducing LC3B aggregation and autophagosome formation in LCSCs. Mechanistically, FSS promoted YAP1 dephosphorylation and transport to the nucleus, which is mediated by RhoA, inducing autophagy. Finally, inhibition of autophagy suppressed cell migration in LCSCs under FSS. In conclusion, FSS promoted the migration of LCSCs via the RhoA-YAP1-autophagy pathway.
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