A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

Article Surgery

SARS-CoV-2-specific humoral and cell-mediated immune responses after immunization with inactivated COVID-19 vaccine in kidney transplant recipients (CVIM 1 study)

Jackrapong Bruminhent et al.

Summary: Solid organ transplant recipients who received inactivated SARS-CoV-2 vaccination showed weak humoral immunity but comparable cell-mediated immunity responses compared to immunocompetent individuals.

AMERICAN JOURNAL OF TRANSPLANTATION (2022)

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Editorial Material Urology & Nephrology

COVID-19 vaccination in kidney transplant recipients

Sophie Caillard et al.

Summary: Kidney transplant recipients, due to therapeutic immunosuppression, have impaired immune responses to the COVID-19 mRNA vaccine, necessitating a personalized intensified vaccination approach for better protection.

NATURE REVIEWS NEPHROLOGY (2021)

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Article Chemistry, Analytical

Ultrasensitive Measurement of Both SARS-CoV-2 RNA and Antibodies from Saliva

Dmitry Ter-Ovanesyan et al.

Summary: This study introduces a highly sensitive method to measure viral RNA and antibodies using saliva samples. By combining viral RNA detection with antibody detection, more infected patients can be accurately identified.

ANALYTICAL CHEMISTRY (2021)

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Article Medicine, Research & Experimental

Single-cell profiling of T and B cell repertoires following SARS-CoV-2 mRNA vaccine

Suhas Sureshchandra et al.

Summary: mRNA vaccines for SARS-CoV-2 have shown strong protection against severe disease, enhancing spike-specific B cells and robust CD4(+) T cell responses. However, CD8(+) T cell responses following vaccination were relatively weak and variable, with clonally expanded CD8(+) T cells observed.

JCI INSIGHT (2021)

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Article Biotechnology & Applied Microbiology

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection

Susanna K. Elledge et al.

Summary: A rapid and low-cost solution-based assay has been developed to detect SARS-CoV-2 antibodies in serum, plasma, whole blood, and to some extent saliva, using split luciferase antibody biosensors. This new method generates quantitative results in 30 minutes, simplifying the complexity and improving the scalability of COVID-19 antibody tests. It is suitable for point-of-care testing, population screening, and use in low-resource settings for monitoring host humoral responses to vaccination or viral infection.

NATURE BIOTECHNOLOGY (2021)

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Article Multidisciplinary Sciences

A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies

Zhong Yao et al.

Summary: A tri-part Nanoluciferase-based assay named SATiN is developed for detecting antibodies against SARS-CoV-2, showing similar sensitivity to ELISA and consistent results with various neutralizing antibody assays. This assay, performed directly in patient sera, offers rapid, quantitative, and cost-effective detection, suggesting potential applications in diagnostics and disease management.

NATURE COMMUNICATIONS (2021)

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Letter Medicine, General & Internal

Antibody Response After a Third Dose of the mRNA-1273 SARS-CoV-2 Vaccine in Kidney Transplant Recipients With Minimal Serologic Response to 2 Doses

Ilies Benotmane et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2021)

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Article Urology & Nephrology

Humoral Response after SARS-CoV-2 mRNA Vaccination in a Cohort of Hemodialysis Patients and Kidney Transplant Recipients

Clement Danthu et al.

Summary: The study shows that kidney transplant recipients have a strong inhibition of humoral response to COVID-19 vaccination due to immunosuppressant therapy, while patients undergoing hemodialysis have lower antibody levels. Factors such as age, serum albumin, and Kt/V can impact the level of antibody response.

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY (2021)

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Article Chemistry, Multidisciplinary

Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

Huahua Yue et al.

Summary: This study presents a CRISPR-Cas12a-based molecular diagnostic technique for amplification-free and absolute quantification of DNA at the single-molecule level, achieving high sensitivity and specificity through optimized reaction parameters and microdroplet technology. The method can directly count diverse viruses' DNAs and demonstrate versatility and quantification capability, showing potential as a versatile and quantitative platform for molecular diagnosis.

NANO LETTERS (2021)

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Review Engineering, Biomedical

CRISPR-based diagnostics

Michael M. Kaminski et al.

Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.

NATURE BIOMEDICAL ENGINEERING (2021)

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Article Immunology

Impaired humoral immunity to SARS-CoV-2 BNT162b2 vaccine in kidney transplant recipients and dialysis patients

Hector Rincon-Arevalo et al.

Summary: Patients with kidney failure are at increased risk for SARS-CoV-2 infection, making effective vaccination critical. However, studies have found significantly impaired antibody responses to the BNT162b2 vaccine in kidney transplant recipients and dialysis patients, leading to insufficient immune responses.

SCIENCE IMMUNOLOGY (2021)

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Review Chemistry, Multidisciplinary

The CRISPR-Cas toolbox for analytical and diagnostic assay development

Yanan Tang et al.

Summary: CRISPR-Cas systems have revolutionized biological and biomedical sciences, particularly in clinical diagnostics and bioimaging. Diverse engineering approaches and better readout platforms are continuously being developed to enhance assay sensitivity and specificity to address disease challenges.

CHEMICAL SOCIETY REVIEWS (2021)

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Article Multidisciplinary Sciences

Longitudinal analysis of antibody decay in convalescent COVID-19 patients

Weiming Xia et al.

Summary: This study analyzed longitudinal blood samples from 943 convalescent COVID-19 patients over seven months to quantify antibody decay rates, finding that patients with higher initial antibody levels showed a significantly faster reduction rate, and most donors' antibodies were detected above threshold for only four months post-infection.

SCIENTIFIC REPORTS (2021)

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Article Medicine, Research & Experimental

Impaired humoral and cellular immunity after SARS-CoV-2 BNT162b2 (tozinameran) prime-boost vaccination in kidney transplant recipients

Arne Sattler et al.

Summary: The study analyzed immune responses in kidney transplant recipients after receiving the BNT162b2 vaccine, finding weaker antibody responses in transplant patients compared to healthy individuals and most hemodialysis patients. Additionally, spike-specific T cell responses were significantly reduced in transplant patients, indicating a need for revised vaccination strategies in immunosuppressed individuals.

JOURNAL OF CLINICAL INVESTIGATION (2021)

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Article Chemistry, Multidisciplinary

Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay

Yongya Li et al.

Summary: The isothermal proximity CRISPR Cas12a assay utilizes the target-induced single-stranded DNase activity of Cas12a for quantitative gene expression profiling and sensitive protein detection. It achieves high sensitivity and specificity through proximity binding instead of recognition by CRISPR RNA, allowing for flexible assay design. This method demonstrated practical applicability by successfully profiling interleukin-6 gene expression during allergen-mediated mast cell activation.

CHEMICAL SCIENCE (2021)

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Article Chemistry, Analytical