期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-31327-y
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资金
- National Key Research and Development Program of China [2021YFA1300503]
- National Natural Science Foundation of China [91940303, 32030064, 31730110, 91753135, 31870814, 32100430]
- CAS Pioneer 100Talent program
- Youth Innovation Promotion Association CAS
- Shanghai Science and Technology Committee Rising-Star Program [19QA1410500]
- China Postdoctoral Science Foundation [2020M681437]
Circular RNAs (circRNAs) have been found to be translated through IRES-driven mechanism. Short elements that drive circRNA translation have been identified, suggesting a pervasive cap-independent translation in the human transcriptome.
Some circular RNAs (circRNAs) were found to be translated through IRES-driven mechanism, however the scope and functions of circRNA translation are unclear because endogenous IRESs are rare. To determine the prevalence and mechanism of circRNA translation, we develop a cell-based system to screen random sequences and identify 97 overrepresented hexamers that drive cap-independent circRNA translation. These IRES-like short elements are significantly enriched in endogenous circRNAs and sufficient to drive circRNA translation. We further identify multiple trans-acting factors that bind these IRES-like elements to initiate translation. Using mass-spectrometry data, hundreds of circRNA-coded peptides are identified, most of which have low abundance due to rapid degradation. As judged by mass-spectrometry, 50% of translatable endogenous circRNAs undergo rolling circle translation, several of which are experimentally validated. Consistently, mutations of the IRES-like element in one circRNA reduce its translation. Collectively, our findings suggest a pervasive translation of circRNAs, providing profound implications in translation control. Unbiased screen of random sequences identified many short IRES-like elements to drive circular RNA translation and hundreds of rolling circle translation events, suggesting a pervasive cap-independent translation in human transcriptome.
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