期刊
CELL DEATH & DISEASE
卷 13, 期 8, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41419-022-05092-1
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资金
- Natural Science Foundation of China [81872188, 81903152]
- Guangdong Science and Technology Project [2017B090901065, 2020A1515010314, 2019B030316003, 2019A1515011200]
- Beijing Bethune Charitable Foundation [flzh202102]
The research revealed the crucial role of radiation-induced paraspeckles in DNA repair and tumor radioresistance, offering a new insight into the ribosome-independent function of ribosomal proteins.
Paraspeckles are mammal-specific membraneless nuclear bodies that participate in various biological processes. NONO, a central paraspeckle component, has been shown to play pivotal roles in DNA double-strand breaks (DSB) repair, whereas its underlying mechanism needs to be further disclosed. Here, using co-immunoprecipitation and mass spectrum, we identified ribosomal protein P0 (RPLP0) as a DSB-induced NONO-binding protein; RPLP0 binds to the RRM1 and RRM2 domains of NONO. Similar to NONO, RPLP0 enhances non-homologous end joining-mediated DSB repair, which was ascribed to a ribosome-independent manner. Interestingly, paraspeckles were induced as early as 15 min after irradiation; it further recruited nuclear RPLP0 to enhance its interaction with NONO. Radiation-induced NONO/RPLP0 complex subsequently anchored at the damaged DNA and increased the autophosphorylation of DNA-PK at Thr2609, thereby enhancing DSB repair. Consistently, in vivo and in vitro experiments showed that depletion of NONO sensitizes tumor cells to radiation. For patients with locally advanced rectal cancer, NONO expression was remarkably increased in tumor tissues and correlated with a poor response to radiochemotherapy. Our findings suggest a pivotal role of radiation-induced paraspeckles in DNA repair and tumor radioresistance, and provide a new insight into the ribosome-independent function of ribosomal proteins.
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