期刊
JOURNAL OF FUNCTIONAL FOODS
卷 95, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2022.105185
关键词
Aronia melanocarpa; Melanoma; B16 cells; Anti -tumor immune response; NK cells; CD8+T lymphocyte
资金
- Ministry of Education, Science and Techno-logical Development [451-03-9/2021-14/200007]
- [323-07-01819/2020-05]
Chokeberry extract (CE) delayed melanoma appearance and increased immune cell infiltration in the tumor microenvironment. CE did not directly affect the melanoma cells, but splenocytes isolated from CE-treated animals showed strong cytotoxic effect on melanoma cells.
Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice. CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1 beta did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-gamma-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-gamma diminished the observed B16 death, sug-gesting that this effect was mediated mainly by splenocyte-derived IFN-gamma. In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-gamma-pro-ducing cells to act against melanoma.
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