期刊
JOURNAL OF FUNCTIONAL FOODS
卷 95, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2022.105150
关键词
High fat diet; Fatty liver; Insulin resistance; Inflammation responds; Metabolic disorder
资金
- Fundamental Research Funds for the Central Universities [45119031C030]
This study demonstrates that Lactobacillus paracasei Jlus66 can effectively improve lipid metabolism disorder, mitochondrial dysfunction, insulin resistance, and inflammatory response in a mouse model of obesity.
Long term high-fat diet (HFD) will lead to obesity and its complications, such as lipid metabolism disorder, insulin resistance and inflammation. The purpose of this study is to demonstrate the mechanism of Lactobacillus paracasei Jlus66 to improve dietary fatty liver in obese mice model. C57BL/6J male mice were randomly divided into three groups (Control, HFD and HFD + Jlus66). Proteins of many pathways related to insulin resistance (IRS-1, AKT, GSK3 beta, GLUT4 and PTP1B), to jejunum permeability (Zo-1, Claudin-1 and Occludin) were detected by using western blotting. Correspondingly, genes of inflammation (Inos, Tnf-alpha and Il-1 beta), mitochondrial dysfunc-tion (Cox5b, Cox7a1, CoxII, Cox8b, Cycs and Sirt1) and oxidative stress (yGcs, Mnsod, Nqo-1, Gpx1 and Ho-1) were detected by using RT-qPCR. As results, Jlus66 effectively improved lipid metabolism disorder and mitochondrial dysfunction, ameliorated insulin resistance and inflammatory response in the liver, jejunum and adipose tissue of HFD-induced obese mice model. Moreover, Jlus66 treated also protected jejunum barrier integrity and alleviated oxidative stress. In conclusion, Jlus66 might be a nutritional strategy for obesity and its complications.
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