Effect of Lactobacillus plantarum LP104 on hyperlipidemia in high-fat diet induced C57BL/6N mice via alteration of intestinal microbiota

The beneficial effects of Lactobacillus plantarum LP104 was investigated on lipid level and the disordered gut microbiota of HFD feeding C57BL/6N mice. C57BL/6N mice were divided into three groups, ordinary diet (Control), high-fat diet (HFD) and HFD with LP104, respectively. After 8 weeks, LP104 alleviated the body weight, liver index, colon length, TC, TG, LDL, TNF alpha and LPS levels. High-throughput sequencing analysis has found that long-term HFD destroy microbial abundance and composition in the gut. Nevertheless, dietary sup-plementation of LP104 reduced the ratio of Firmicutes to Bacteroides and regulate the gut microbiota community structure positively. Meanwhile, the intake of LP104 also increased the abundance of beneficial bacteria Bifi-dobacterium in the cecum, which improves the intestinal flora disorder induced by a high-fat diet. The present study shows that LP104 supplementation improved the amelioration HFD induced hyperlipidemia by positively regulating intestinal microorganisms.

Automated summary

The beneficial effects of Lactobacillus plantarum LP104 on lipid level and disordered gut microbiota in mice fed a high-fat diet were investigated in this study. The results showed that LP104 supplementation could alleviate body weight gain, liver index increase, colon length reduction, as well as improve TC, TG, LDL, TNF alpha, and LPS levels. High-throughput sequencing analysis revealed that long-term high-fat diet negatively affected microbial abundance and composition in the gut, but LP104 supplementation reduced the ratio of Firmicutes to Bacteroides and positively regulated the gut microbiota community structure. Additionally, the intake of LP104 also increased the abundance of beneficial bacteria Bifidobacterium in the cecum, which improved the intestinal flora disorder induced by a high-fat diet. These findings suggest that LP104 supplementation can improve high-fat diet-induced hyperlipidemia by positively regulating intestinal microorganisms.