期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 109, 期 4, 页码 1683-1690出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jee/tow090
关键词
Diabrotica virgifera virgifera; Zea mays; methyl anthranilate; host location; repellent
类别
资金
- Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2014-67013-21764]
- Colorado Agricultural Experiment Station [622]
- NIFA [687092, 2014-67013-21764] Funding Source: Federal RePORTER
Methyl anthranilate was identified as the active compound in extracts of maize (Zea mays L.) roots that were shown to be repellent to neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae. A bio-assay-driven approach was used to isolate the active material from diethyl ether extracts of roots from germinating maize seeds. Separation of the extract on a Florisil column yielded an active fraction of 90: 10 hexane: diethyl ether. Analysis with gas chromatography-mass spectrometry identified two compounds in the active fraction: indole (2,3-benzopyrrole) and methyl anthranilate (methyl 2-aminobenzoate). When tested in behavioral bioassays, methyl anthranilate elicited a significant (P < 0.05) repellent response at doses of 1, 10, and 100 mu g. In subsequent single-choice bioassays, 1, 10, and 100 mu g of methyl anthranilate prevented larvae from approaching 10 mmol/mol concentrations of carbon dioxide, which is normally highly attractive to the larvae. Indole, the other compound identified from the active fraction, did not elicit a behavioral response by the larvae. Methyl anthranilate has potential for development as a management tool for western corn rootworm larvae and may be best suited for use in a push-pull control strategy.
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