期刊
VIRUSES-BASEL
卷 14, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v14081782
关键词
PEDV; ATM; gamma H2AX; caspase; DNA damage response
类别
资金
- National Natural Science Foundation [31900141, 31472218]
- Fundamental Research Funds for the Central Universities [KJQN202022]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
This study reveals that the porcine epidemic diarrhea virus (PEDV) induces DNA damage response (DDR) through the ROS-ATM and caspase7-CAD-gamma H2AX signaling pathways, which facilitates its early replication.
DNA damage response (DDR) is an evolutionarily conserved mechanism by which eukaryotic cells sense DNA lesions caused by intrinsic and extrinsic stimuli, including virus infection. Although interactions between DNA viruses and DDR have been extensively studied, how RNA viruses, especially coronaviruses, regulate DDR remains unknown. A previous study showed that the porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus in the Coronaviridae family, induces DDR in infected cells. However, the underlying mechanism was unclear. This study showed that PEDV activates the ATM-Chk2 signaling, while inhibition of ATM or Chk2 dampens the early stage of PEDV infection. Additionally, we found that PEDV-activated ATM signaling correlates with intracellular ROS production. Interestingly, we showed that, unlike the typical gamma H2AX foci, PEDV infection leads to a unique gamma H2AX staining pattern, including phase I (nuclear ring staining), II (pan-nuclear staining), and III (co-staining with apoptotic bodies), which highly resembles the apoptosis process. Furthermore, we demonstrated that PEDV-induced H2AX phosphorylation depends on the activation of caspase-7 and caspase-activated DNAse (CAD), but not ATM-Chk2. Finally, we showed that the knockdown of H2AX attenuates PEDV replication. Taken together, we conclude that PEDV induces DDR through the ROS-ATM and caspase7-CAD-gamma H2AX signaling pathways to foster its early replication.
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