4.6 Article

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

期刊

VIRUSES-BASEL
卷 14, 期 7, 页码 -

出版社

MDPI
DOI: 10.3390/v14071518

关键词

bacteriophages; Tevenvirinae subfamily; myoviruses; Enterobacter cloacae; antibiotic resistance; phage stability; urinary tract infection

类别

资金

  1. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy at the Polish Academy of Sciences

向作者/读者索取更多资源

In this study, two newly isolated myoviruses that can lyse multidrug-resistant Enterobacter spp. were characterized. These phages belong to a poorly represented phage group and show potential for therapeutic use against urinary tract infections.
In an era of antibiotic therapy crisis caused by spreading antimicrobial resistance, and when recurrent urinary tract infections constitute a serious social and medical problem, the isolation and complex characterization of phages with a potential therapeutic application represents a promising solution. It is an inevitable, and even a necessary direction in the development of current phage research. In this paper, we present two newly isolated myoviruses that show lytic activity against multidrug-resistant clinical isolates of Enterobacter spp. (E. cloacae, E. hormaechei, and E. kobei), the genomes of which belong to a poorly represented phage group. Both phages were classified as part of the Tevenvirinae subfamily (Entb_43 was recognized as Karamvirus and Entb_45 as Kanagawavirus). Phage lytic spectra ranging from 40 to 60% were obtained. The most effective phage-to-bacteria ratios (MOI = 0.01 and MOI = 0.001) for both the phage amplification and their lytic activity against planktonic bacteria were also estimated. Complete adsorption to host cells were obtained after about 20 min for Entb_43 and 10 min for Entb_45. The phage lysates retained their initial titers even during six months of storage at both -70 degrees C and 4 degrees C, whereas storage at 37 degrees C caused a complete loss in their activity. We showed that phages retained their activity after incubation with solutions of silver and copper nanoparticles, which may indicate possible synergistic antibacterial activity. Moreover, a significant reduction in phage titers was observed after incubation with a disinfectant containing octenidinum dihydrochloridum and phenoxyethanol, as well as with 70% ethanol. The observed maintenance of phage activity during incubation in a urine sample, along with other described properties, may suggest a therapeutic potential of phages at the infection site after intravesical administration.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据