4.5 Article

Protective immunity against spring viremia of carp virus by mannose modified chitosan loaded DNA vaccine

期刊

VIRUS RESEARCH
卷 320, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.virusres.2022.198896

关键词

Mannose; Chitosan; DNA vaccine; Immune response; Spring viremia of carp virus

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资金

  1. Natural Science Foundation of China [32173011]

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This study developed a mannose-modified chitosan-encapsulated DNA vaccine that effectively combats SVCV, inducing a strong immune response and providing substantial protection. This could be beneficial for the development of more aquatic animal vaccines in the future.
Spring viremia of carp virus (SVCV) usually be considered as one of the serious in viral diseases of aquaculture, and DNA vaccine with novel delivery mechanism or adjuvant has proven to be a promising and effective strategy to control aquatic animal diseases. In this study, the mannose-modified chitosan, a carrier system for vaccine delivery, were used to developed a chitosan-encapsulated DNA vaccine (CS-M-G) against SVCV, then investigated immune response induced by the vaccine. Our results showed that CS-M-G was confirmed the spherical or elliptical with even distribution and ranging from approximately 50 to 150 nm in size, the expression of the antigen gene could still be detected after 21 d post vaccination. The CS-M-G induces the highest antibody levels in the 20 mu g dose group which is about 3 times than naked plasmid group at 21 d post vaccination, and still hold a higher level than control group at 28 d post vaccination. On the side, strongest protection with relative percent survival of 62.1% in the 20 mu g CS-M-G group, which could produce significantly higher enzyme activities and upregulated expression of immune-associated genes than control group. Thus, our results indicate that DNA vaccine loaded with mannose-modified chitosan induces strong immune response and provided an effective protection against SVCV infection, may be helpful and extended for developing more aquatic animal vaccines in the future.

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