The homologous region hr4a of Autographa californica multiple nucleopolyhedrovirus specifically enhances viral early promoters

Autographa californica multiple nucleocapsid polyhedrosis virus (AcMNPV) genome contains several homologous regions (hrs) which can be used as origins of viral DNA replication and transcriptional enhancers. Early studies have found that some hrs can not only stimulate viral early promoters, but also enhance gene expression driven by viral late promoters. In this study, by adding hr4a to the upstream of different types of viral promoters and enhanced green fluorescent protein (egfp) reporter gene, we investigated the enhancement effect of hr4a on eGFP expression. The results showed that hr4a increased the gene transcription controlled by early promoters, but had no enhancer effect on late promoters.

Automated summary

In this study, the enhancement effect of hr4a on gene expression was investigated by adding hr4a to different types of viral promoters and the reporter gene egfp. The results showed that hr4a increased gene transcription controlled by early promoters, but had no enhancer effect on late promoters.