期刊
ULTRASONICS SONOCHEMISTRY
卷 88, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ultsonch.2022.106062
关键词
Maca polysaccharide; Ultrasonic-assisted extraction; Purification; Macrophage; Immunoregulatory activity
资金
- Key Research & Development Pro-gram of Jiangsu Province, China [BE2018368]
- Graduate Research and Innovative Projects of Jiangsu Province, China [KYCX20_3045]
This study evaluates the effect of multimodal ultrasound on the extraction efficiency and immunoregulatory activity of polysaccharides from Lepidium meyenii Walp. The results showed that multimodal ultrasound improved the extraction rates of purified polysaccharides and increased their immune activity. This has significant implications for using these polysaccharides in food or medicine.
This study evaluates the effect of multimodal ultrasound on the extraction efficiency and immunoregulatory activity of polysaccharides from Lepidium meyenii Walp. (LMP). The separation and purification of maca polysaccharides were investigated by the DEAE-52 cellulose column, and the monosaccharide compositions were identified by HPGPC. Their immune activity was analyzed by the secretion of cytokines (TNF-alpha and IL-6) from RAW 264.7 macrophage. The results showed that the optimal extraction conditions were energy aggregation alternation dual-frequency ultrasound (EADU) with frequency combinations of 20/35, extraction time of 15 min, material/water ratio of 1:10 g/mL, ultrasonic power intensity of 150 W/L, intermittent time ratio of 4 s/3 s, and extraction temperature of 50 celcius. The extraction rates of purified polysaccharides (US3) increased by 44.90%. The LMP extracted by EADU contained arabinose, galactose, and glucose in the molar ratios of 2.9:2.72:5.05. In addition, US3 promoted the release of TNF-alpha and IL-6 from RAW 264.7 better than RS3 (purified polysaccharides extracted by hot water), which indicated that US3 exerted remarkable immune activity. It could be an excellent functional additive in food or medicine.
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