4.7 Article

Ca2+ signaling-mediated low-intensity pulsed ultrasound-induced proliferation and activation of motor neuron cells

期刊

ULTRASONICS
卷 124, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.ultras.2022.106739

关键词

Low-intensity pulsed ultrasound; Motor neuron disease; NSC-34 cell line; Neurite outgrowth; Morphology; Ca (2+) signaling; Ca (2+) -dependent transcription factors

资金

  1. Ministry of Science and Technology of Taiwan [110-2221-E-006-035-MY3, 110-2320-B-006-060]

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This study investigated the effect of low-intensity pulsed ultrasound (LIPUS) on the NSC-34 cell line, a model for motor neuron diseases (MND). The findings suggest that LIPUS stimulation can promote the maturation and proliferation of NSC-34 cells, potentially through the activation of Ca2+ signaling and transcription factors.
Motor neuron diseases (MND) including amyotrophic lateral sclerosis and Parkinson disease are commonly neurodegenerative, causing a gradual loss of nerve cells and affecting the mechanisms underlying changes in calcium (Ca2+)-regulated dendritic growth. In this study, the NSC-34 cell line, a population of hybridomas generated using mouse spinal cord cells with neuroblastoma, was used to investigate the effect of low-intensity pulsed ultrasound (LIPUS) as part of an MND treatment model. After NSC-34 cells were seeded for 24 h, LIPUS stimulation was performed on the cells at days 1 and 3 using a non-focused transducer at 1.15 MHz for 8 min. NSC-34 cell proliferation and morphological changes were observed at various LIPUS intensities and different combinations of Ca2+ channel blockers. The nuclear translocation of Ca2+-dependent transcription factors was also examined. We observed that the neurite outgrowth and cell number of NSC-34 significantly increased with LIPUS stimulation at days 2 and 4, which may be associated with the treatment's positive effect on the activation of Ca2+-dependent transcription factors, such as nuclear factor of activated T cells and nuclear factor-kappa B. Our findings suggest that the LIPUS-induced Ca2+ signaling and transcription factor activation facilitate the morphological maturation and proliferation of NSC-34 cells, presenting a promising noninvasive method to improve stimulation therapy for MNDs in the future.

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