4.7 Review

Mass spectrometry analysis of S-nitrosylation of proteins and its role in cancer, cardiovascular and neurodegenerative diseases

期刊

TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 152, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2022.116625

关键词

Protein S-Nitrosylation; Mass spectrometry; Proteomics; Cancer; Cardiovascular diseases; Neurodegenerative diseases

资金

  1. National Natural Science Foundation of China [22104081]
  2. Shanghai Pujiang Program [20PJ1406300]
  3. Interdisciplinary Program of Shanghai Jiao Tong University [YG2021QN45]

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This review summarizes the application of mass spectrometry techniques in the analysis of protein S-nitrosylation, including sample preparation techniques and qualitative and quantitative strategies. The roles of protein S-nitrosylation in human diseases are discussed, with an emphasis on current limitations and future research directions.
Mass spectrometry (MS) has become an indispensable tool in the study of protein identification, protein interactions, and post-translational modifications (PTMs). S-nitrosylation is a major nitric oxide-dependent covalent PTM that targets specific cysteine residues and affects a variety of processes in diverse biological systems. Nowadays, the significance of protein S-nitrosylation in cellular physiology and pathophysiology is increasingly recognized. To deeply understand the formation, selectivity, and regulatory roles of S-nitrosylation, global identifications of S-nitrosylated proteins and their modification sites are highly required. This review summarizes the currently available approaches combining with MS for the analysis of protein S-nitrosylation. Sample preparation techniques specific to S-nitrosylated proteins are evaluated. Meanwhile, we also demonstrate the MS-based qualitative and quantitative proteomic strategies for S-nitrosylation measurement. Roles of protein S-nitrosylation in human diseases are further discussed with emphasis on cancer, cardiovascular and neurodegenerative diseases. In the end, current limitations and future research directions are presented. (c) 2022 Elsevier B.V. All rights reserved.

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