期刊
THERIOGENOLOGY
卷 186, 期 -, 页码 146-154出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2022.04.007
关键词
Ovary; Egg-ceasing; MicroRNA; High throughput sequencing; Reproduction regulation
资金
- National Natural Science Foundation of China [31660663]
- National Key Research and Development Program [2016YFD0500510]
This study identified differentially expressed miRNAs and mRNAs involved in ovarian atrophy through high throughput sequencing. Functional analysis and interaction network construction revealed key regulators in ovary development. These findings expanded our understanding of the molecular mechanism underlying ovarian atrophy.
Egg-ceasing is a phenomenon that occurs in most avian species and significantly reduces productivity. Although several factors are reported to regulate the reproduction progress, the underlying molecular mechanism of egg-ceasing remains obscure. Herein, we identified and explored the differentially expressed miRNAs and mRNAs involved in ovarian atrophy via high throughput sequencing. We identified a total of 901 mRNAs and 50 miRNAs that were differentially expressed in egg-laying and atrophic ovaries. Among them, numerous differentially expressed gene (DEG) transcripts and target genes for miRNAs were significantly enriched in Gene Ontology terms such as reproductive processes, cell proliferation, and apoptosis pathways. In addition, an interaction network was constructed by considering target relationships and correlation of the expression levels between ovary development-related genes, miRNAs and pathways. We discovered mRNA and miRNAs transcripts that are candidate regulators of ovary development in egg-ceased geese. Our findings expanded our understanding of the functional of miRNAs in ovarian atrophy and demonstrated that RNA-Seq is a powerful tool for examining the molecular mechanism in regulating egg-ceasing.(c) 2022 Elsevier Inc. All rights reserved.
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