4.7 Article

Isothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs

期刊

TALANTA
卷 246, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.talanta.2022.123502

关键词

Gastric cancer; microRNAs Lateral flow biosensor; Rolling circle amplification; In vitro diagnosis

资金

  1. National R&D Programs through the National Research Foundation (NRF) of Korea - Ministry of Science and ICT (MSIT) of Korea [NRF-2021M3E5E3080379, NRF2021M3H4A1A02051048, NRF-2018M3A9E2022821, NRF2021M3E5E3080844, NRF-2022R1C1C1008815, NRF2020R1A2C1010453]
  2. Korean Fund for Regenerative Medicine (KFRM) - Korean government (the Ministry of Science and ICT) [21A0404L1]
  3. Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) - Ministry of Environment (ME) of Korea [2021003370003, 20009121]
  4. Ministry of Trade, Industry & Energy (MOTIE) of Korea [CSM2105M101]
  5. KRIBB Research Initiative Program [1711134081, 1711134038]

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Researchers have developed a simple and sensitive in vitro diagnostic system for detecting gastric cancer-related biomarkers. The system combines isothermal amplification and lateral flow biosensor to detect target miRNAs with excellent sensitivity and selectivity within 3 hours. This technology is of great importance for the early diagnosis and prognosis monitoring of gastric cancer and can also be applied to detect biomarkers related to other diseases.
MicroRNAs (miRNAs) are important diagnostic and prognostic biomarkers for various tumors. Currently, many diagnostic systems have been developed to detect miRNAs, but simple techniques for detecting miRNAs are still required. Recently, we reported that the expression of miRNA-135b is upregulated in gastric epithelial cells during gastric inflammation and carcinogenesis. Our aim was to develop an in vitro diagnostic platform to analyze the expression of gastric cancer-related biomarkers in the blood. The diagnostic platform comprised an isothermal amplification-based lateral flow biosensor (IA-LFB) that enables easy diagnosis of gastric cancer through visual observation. In this platform, trace amounts of biomarkers are isothermally amplified through rolling circle amplification (RCA), and the amplified product is grafted to the LFB. The performance of the IA-LFB was confirmed using RNAs extracted from in vitro and in vivo models. The platform could detect target miRNAs within 3 h with excellent sensitivity and selectivity. In particular, the IA-LFB could detect the overexpression of gastric cancer-related markers (miRNA-135b and miRNA-21) in RNAs extracted from the blood of patients with various stages (stages 1-4) of gastric cancer compared to that in healthy volunteers. Therefore, IA-LFB is a simple and sensitive in vitro diagnostic system for detecting gastric cancer-related biomarkers and can contribute to the early diagnosis and prognosis monitoring of gastric cancer. Furthermore, this technology can be applied to systems that can detect multiple biomarkers related to various diseases (such as infectious and genetic diseases).

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