Microfluidic acoustic valve for capturing locomotive microorganism without anesthesia

The capture of locomotive small organisms is challenging but interesting. In this study, we developed a method to capture Paramecium with acoustic valve. Constructed by the focused surface acoustic waves (FSAW), acoustic valve can be used to block the dispersed phase in liquid flow. Under the joint action of acoustic radiation force and drag force, Paramecium can be successfully confined in a narrow space in the channel without losing its movement ability. By adjusting the applied voltage and the inlet pressure, the Paramecium can be allowed to pass, but the Paramecium cysts get captured. This acoustic device is potential in biochemical experiments, providing a new method for capturing or sorting micro swimmers.

Automated summary

In this study, a method was developed to capture Paramecium using an acoustic valve. By adjusting the voltage and inlet pressure, Paramecium can be confined in a narrow space without losing its movement ability. This method has the potential for capturing or sorting micro swimmers in biochemical experiments.