Bacteriophage cocktail as a promising bio-enhancer for methanogenic activities in anaerobic membrane bioreactors

This study aimed to explore the effect of a bacteriophage cocktail, pyophage, on the treatment of wastewater contain-ing antibiotics in an anaerobic membrane bioreactor (AnMBR). During the operational period, performance of the AnMBR was monitored through the changes in chemical oxygen demand (COD), antibiotic removal, transmembrane pressure, and biogas production. Microbial community structure and composition, as well as the occurrence of antibi-otic resistance genes were analyzed through shotgun metagenomics analysis. When exposed to pyophage, COD re-moval efficiency was enhanced up to 96%, whereas membrane fouling was delayed by 25%. Average biogas production was doubled from 224.2 mL/d in control with antibiotics to 447.3 mL/d when exposed to pyophage cock-tail with considerable alterations to the archaeal and bacterial community structures. Most notably, the methanogenic community shifted from dominance of Methanothermobacter to Methanoculleus, along with syntrophic bacteria. The results provide insight into the synergistic effects of phage-bacteria and methanogenic communities and illustrate the potential of bacteriophages as bio-enhancers.

Automated summary

This study explored the effect of a bacteriophage cocktail, pyophage, on the treatment of wastewater containing antibiotics in an anaerobic membrane bioreactor. The results showed that pyophage improved COD removal efficiency, delayed membrane fouling, and promoted biogas production through alterations to the microbial community structure and composition.