期刊
SCIENCE
卷 376, 期 6600, 页码 1431-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abh2841
关键词
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资金
- Wellcome Trust Principal Research Fellowship [223103/Z/21/Z]
- NIH MERIT award [R37 AI054503]
- Medical Research Council UK [MC_U105663142]
- Wellcome Trust Investigator award [110159/A/15/Z]
- MCIN
- ESF Investing in your Future Fellowship [RYC2019-027799-I/AEI/10.13039/501100011033]
- Wellcome Trust [223103/Z/21/Z] Funding Source: Wellcome Trust
Excess TNF in mycobacterium-infected macrophages increases mitochondrial reactive oxygen species (mROS), causing susceptibility to tuberculosis. The study identifies the mechanism of TNF-induced mROS through reverse electron transport. The use of the drug metformin prevents TNF-induced mROS and necrosis in tuberculosis.
Tumor necrosis factor (TNF) is a critical host resistance factor against tuberculosis. However, excess TNF produces susceptibility by increasing mitochondrial reactive oxygen species (mROS), which initiate a signaling cascade to cause pathogenic necrosis of mycobacterium-infected macrophages. In zebrafish, we identified the mechanism of TNF-induced mROS in tuberculosis. Excess TNF in mycobacterium-infected macrophages elevates mROS production by reverse electron transport (RET) through complex I. TNF-activated cellular glutamine uptake leads to an increased concentration of succinate, a Krebs cycle intermediate. Oxidation of this elevated succinate by complex II drives RET, thereby generating the mROS superoxide at complex I. The complex I inhibitor metformin, a widely used antidiabetic drug, prevents TNF-induced mROS and necrosis of Mycobacterium tuberculosis-infected zebrafish and human macrophages; metformin may therefore be useful in tuberculosis therapy.
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