期刊
RNA
卷 28, 期 9, 页码 1185-1196出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.079131.122
关键词
rRNA; modified nucleosides; Bacillus subtilis; methyltransferase
资金
- Fonds J. Brachet
- French National Program Investissement d'Avenir (Labex NetRNA) [ANR-10-LABX-0036_NETRNA]
This study demonstrates that the ysgA gene in Bacillus subtilis encodes a 2'-O-methyltransferase that targets the G2553 position of the A-loop of 23S rRNA. The enzyme modifies free 23S rRNA during early ribosome biogenesis without affecting the assembly of ribosome subunits.
A previous bioinformatic analysis predicted that the ysgA open reading frame of Bacillus subtilis encodes an RNA methyltransferase of the SPOUT superfamily. Here we show that YsgA is the 2'-O-methyltransferase that targets position G2553 (Escherichia coli numbering) of the A-loop of 23S rRNA. This was shown by a combination of biochemical and mass spectrometry approaches using both rRNA extracted from B. subtilis wild-type or.ysgA cells and in vitro synthesized rRNA. When the target G2553 is mutated, YsgA is able to methylate the ribose of adenosine. However, it cannot methylate cytidine nor uridine. The enzyme modifies free 23S rRNA but not the fully assembled ribosome nor the 50S subunit, suggesting that the modification occurs early during ribosome biogenesis. Nevertheless, ribosome subunits assembly is unaffected in a B. subtilis Delta ysgA mutant strain. The crystal structure of the recombinant YsgA protein, combined with mutagenesis data, outlined in this article highlights a typical SPOUT fold preceded by an L7Ae/L30 (eL8/eL30 in a new nomenclature) amino-terminal domain.
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