期刊
REPRODUCTIVE BIOMEDICINE ONLINE
卷 45, 期 6, 页码 1085-1096出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2022.06.003
关键词
Crown rump length; Culture media; Embryonic development; Fetal weight; IVF
This study demonstrates that the SAGE 1-Step culture medium accelerates embryonic growth trajectories compared to Vitrolife G-1 PLUS and naturally conceived pregnancies, particularly in male embryos. Further research should focus on the impact of culture media on postnatal development and susceptibility to non-communicable diseases.
Research question: Are there (sex-specific) differences in first-trimester embryonic growth and morphological development between two culture media used for IVF and intracytoplasmic sperm injection (ICSI) treatment?Design: A total of 835 singleton pregnancies from a prospective hospital-based cohort study were included, of which 153 conceived after IVF/ICSI treatment with Vitrolife G-1TM PLUS culture medium, 252 after culture in SAGE 1-StepTM and 430 were naturally conceived. Longitudinal three-dimensional ultrasound examinations were performed at 7, 9 and 11 weeks of gestation for offline biometric (crown rump length, CRL), volumetric (embryonic volume) and morphological (Carnegie stage) measurements.Results: Embryos cultured in SAGE 1-Step grew faster than those cultured in Vitrolife G-1 PLUS (betaEV 0.030 3 root ml [95% CI 0.008-0.052], P = 0.007). After stratification for fetal sex, male embryos cultured in SAGE 1-Step demonstrated faster growth than those cultured in Vitrolife G-1 PLUS (betaEV 0.048 3 root ml [95% CI 0.015-0.081], P = 0.005). When compared with naturally conceived embryos, those cultured in SAGE 1-Step grew faster (betaEV 0.040 3 root ml [95% CI 0.012-0.069], P = 0.005). This association was most pronounced in male embryos (betaEV 0.078 3 root ml [95% CI 0.035-0.120], P < 0.001).Conclusions: This study shows that SAGE 1-Step culture medium accelerates embryonic growth trajectories compared with Vitrolife G-1 PLUS and naturally conceived pregnancies, especially in male embryos. Further research should focus on the impact of culture media on postnatal development and the susceptibility to non-communicable diseases.
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