Isolation, identification, and pathogenicity of a ALV-K strain from Chinese indigenous chicken breed

Subgroup K avian leukosis virus (ALV-K) is a new subgroup of avian leukosis virus (ALV) first identified in Chinese indigenous chickens in recent years. In this study, an ALV-K strain was isolated from Luhua chicken in Shandong province, China, and designated SD20LH01. The full-length genomic sequence of SD20LH01 was 7491 bp, which had the highest homology with ALV-K reference strains GDFX0601, GDFX0602 and GDFX0603. The nucleotide homology of env gene of SD20LH01 with reference strains of subgroup A, B, C, D, E, and J was ranged from 57.1 to 93.2%, while 94.1 to 99.4% with other ALV-K reference strains. The nucleotide difference of SD20LH01 mainly clustered with gp85 gene and U3 sequence when compared with the reference strain of ALV-K. In order to investigate the pathogenicity of SD20LH01, SPF chicken embryos were infected by yolk sac inoculation, and 1-day-old chickens were infected by intraperitoneal inoculation of SD20LH01. The results showed that yolk sac inoculation of SD20LH01 could induce persistent viremia, growth retardation and reduce the immune response to NDV and AIV-H9 vaccines. However, intra-peritoneal inoculation in 1-day-old chickens could only induce a low level of viremia. In addition, no tumors were found in infected chickens during the animal experiments. This study enriched the genomic sequence data of ALV-K isolated in Chinese indigenous chickens, and laid a foundation for further study on the pathogenesis and prevention of ALV-K.

Automated summary

In this study, an ALV-K strain SD20LH01 was isolated from Chinese indigenous chickens. The full-length genomic sequence of SD20LH01 showed high homology with ALV-K reference strains GDFX0601, GDFX0602, and GDFX0603, with nucleotide differences mainly in the gp85 gene and U3 sequence. The pathogenicity of SD20LH01 was investigated using yolk sac and intraperitoneal inoculation methods, which induced different levels of viremia and immune response in chicken embryos and 1-day-old chickens. No tumors were found in infected chickens. This study contributes to the understanding of ALV-K in Chinese indigenous chickens and provides a foundation for further research on its pathogenesis and prevention.