期刊
JOURNAL OF DERMATOLOGICAL SCIENCE
卷 84, 期 3, 页码 248-257出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jdermsci.2016.09.011
关键词
Atopic dermatitis; Dermal dendritic cell; Epicutaneous immunization; Th1 response
类别
资金
- Ministry of Science and Technology [MOST 103-2314-B-002-155-MY2]
Background: Epicutaneous immunization with allergens is an important sensitization route for atopic dermatitis. We recently showed in addition to the Th2 response following single epicutaneous immunization, a remarkable Thl response is induced in B6 mice, but not in BALB/c mice, mimicking the immune response to allergens in human non-atopics and atopics. Objective: We investigated the underlying mechanisms driving this differential Thl response between BALB/c and B6 mice. Methods: We characterized dermal dendritic cells by flow cytometric analysis. We measured the induced Th1/Th2 responses by measuring the IFN-gamma/IL-13 contents of supernatants of antigen reactivation cultures of lymph node cells. Results: We demonstrate that more dermal dendritic cells with higher activation status migrate into draining lymph nodes of B6 mice compared to BALB/c mice. Dermal dendritic cells of B6 mice have a greater ability to capture protein antigen than those of BALB/c mice. Moreover, increasing the activation status or amount of captured antigen in dermal dendritic cells induced a Thl response in BALB/c mice. Further, differential activation behavior, but not antigen-capturing ability of dermal dendritic cells between BALB/c and B6 mice is dendritic cell-intrinsic. Conclusion: These results show that the differential activation behavior of dermal dendritic cells underlies the strain-specific Thl responses following single epicutaneous immunization. Furthermore, our findings highlight the potential differences between human atopics and non-atopics and provide useful information for the prediction and prevention of atopic diseases. (C) 2016 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
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