4.6 Article

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

期刊

PLOS ONE
卷 17, 期 7, 页码 -

出版社

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0271553

关键词

-

资金

  1. Cholangiocarcinoma Research Institute, Khon Kaen University, and Fluke-Free Thailand
  2. National Research Council of Thailand
  3. Thailand Center of Excellence for Life Sciences (TCELS)
  4. National Science, Research and Innovation Fund (NSRF) under the Basic Research Fund of Khon Kaen University through Cholangiocarcinoma Research Institute [CARIBRF64-50]
  5. Sir Henry Wellcome Postdoctoral Fellowship
  6. Wellcome Trust [215919/Z/19/Z]
  7. Wellcome Trust [215919/Z/19/Z] Funding Source: Wellcome Trust

向作者/读者索取更多资源

Antigen detection in urine using ELISA is more sensitive than fecal examination for diagnosis and assessment of drug treatment in opisthorchiasis. There is a significant positive correlation between the concentration of antigen in urine and fecal egg counts. The urine assay has high diagnostic performance and minimal daily variation, making it useful for diagnosis and population screening of opisthorchiasis.
Antigen detection in urine using an enzyme-linked immunosorbent assay (ELISA) is more sensitive than fecal examination for diagnosis of opisthorchiasis and for assessment of the effects of drug treatment. It is not known whether day-to-day variation of urine composition, including levels of Opisthorchis viverrini antigen, influences the urine assay. We investigated this topic with the cooperation of participants from two localities in Northeast Thailand. Project participants were screened for parasite infections for three consecutive days using the quantitative formalin-ethyl acetate concentration technique (FECT) to detect O. viverrini eggs and the urine ELISA for detection of O. viverrini antigen. A subset of participants (n = 801) with matched fecal and urine samples were analyzed for comparison of inter-day prevalence estimates and the performance of the urine assay compared against FECT for diagnosis of opisthorchiasis. The daily prevalence measured by the urine assay ranged between 29.0%-30.2% while those by FECT ranged between 11.9%-20.2%. The cumulative three-day prevalence estimate determined by the urine antigen assay was 30.3%, which was significantly higher than that by FECT (20.2%, p < 0.05). A significant positive correlation was found between the concentration of antigen in urine and fecal egg counts (p < 0.001). Overall, the urine assay had better diagnostic performance for opisthorchiasis than fecal examination by FECT. The high sensitivity plus negligible daily variation of O. viverrini antigen in urine indicates the utility of the urine assay for diagnosis, as well as population screening, of opisthorchiasis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据