Comparative metabolite profiling of rice contrasts reveal combined drought and heat stress signatures in flag leaf and spikelets

Under semi-irrigated ecosystem, rice is often exposed to a combination of drought and heat stress, especially at the reproductive stage, leading to substantial yield loss. Combined stress studies are very limited in rice partly due to the difficulty in creating heat stress on a larger scale. Here, 24 cultivars with specific stress adaptive traits were phenotyped for spikelet sterility under combined stress using the natural summer temperatures and open drought phenotyping facility, simulating the field conditions. LC-MS/MS based metabolite profiling was performed in flag leaves and spikelets of three cultivars contrasting for spikelet sterility and source (leaf weight) treated to drought, heat and combined stress. Constitutively regulated metabolites, metabolic signatures common to all stresses, cultivars and tissues, metabolites common to both the tissues across the stresses and cultivars and metabolites common to each cultivar across the tissues and stresses were identified. Under combined stress, metabolites differentially accumulated between cultivars contrasting for spikelet sterility but similar for source and cultivars contrasting for both spikelet sterility and source have been identified. These metabolites would serve as markers towards improving combined stress tolerance of rice.

Automated summary

Under semi-irrigated ecosystem, rice faces a combination of drought and heat stress at the reproductive stage, leading to yield loss. This study analyzed the metabolite profiles in flag leaves and spikelets of rice cultivars under drought, heat, and combined stress. Metabolites differentially accumulated between cultivars with contrasting spikelet sterility were identified, which can be used as markers for improving combined stress tolerance in rice.