期刊
PHYTOTHERAPY RESEARCH
卷 36, 期 9, 页码 3555-3570出版社
WILEY
DOI: 10.1002/ptr.7523
关键词
AKT; colorectal cancer; GLI1; non-canonical hedgehog signaling pathway; ursolic acid
资金
- Chengdu University of Traditional Chinese Medicine [GJJJ2021003]
- Sichuan Provincial Administration of Traditional Chinese Medicine of China [2021MS447, 2021XYCZ007]
- Health Commission of Sichuan Province of China [21PJ107]
- National Natural Science Foundation of China [81173598, 81573813]
It has been discovered that ursolic acid (UA), a compound found in medicinal herbs, has potent effects against colorectal cancer (CRC) by inhibiting non-canonical Hedgehog signaling. This study investigated the influence of UA on non-canonical Hedgehog signaling in CRC, and found that UA inhibited cell proliferation, induced apoptosis, and down-regulated signaling proteins in CRC cells. Furthermore, UA also improved symptoms, inhibited tumor growth, and induced apoptosis in a CRC xenograft model.
It is being brought to light that smoothened (SMO)-independent non-canonical Hedgehog signaling is associated with the pathogenesis of various cancers. Ursolic acid (UA), a pentacyclic triterpenoid present in many medicinal herbs, manifests potent effectiveness against multiple malignancies including colorectal cancer (CRC). In our previous study, UA was found to protect against CRC in vitro by suppression of canonical Hedgehog signaling cascade. Here, the influence of UA on SMO-independent non-canonical Hedgehog signaling in CRC was investigated in the present study, which demonstrated that UA hampered the proliferation and migration, induced the apoptosis of HCT-116(hSMO-) cells with SMO gene knockdown, accompanied by the augmented expression of the suppressor of fused (SUFU), and lessened levels of MYC (c-Myc), glioma-associated oncogene (GLI1) and Sonic Hedgehog (SHH), and lowered phosphorylation of protein kinase B (PKB, AKT), suggesting that UA diminished non-canonical Hedgehog signal transduction in CRC. In HCT-116(hSMO-) xenograft tumor, UA ameliorated the symptoms, impeded the growth and caused the apoptosis of CRC, with heightened SUFU expression, and abated levels of MYC, GLI1, and SHH, and mitigated phosphorylation of AKT, indicating that UA down-regulated non-canonical Hedgehog signaling cascade in CRC. Taken together, UA may alleviate CRC by suppressing AKT signaling-dependent activation of SMO-independent non-canonical Hedgehog pathway.
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