期刊
PHYTOMEDICINE
卷 102, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2022.154154
关键词
SARS-CoV-2; Traditional Chinese Medicine; Polygoni Multiflori Radix; S-protein ELISA; 3CL protease; Pseudovirus tests
资金
- Hong Kong Government Food and Health Bureau [HMRF20SC07[COVID190213]]
- Key-Area Research and Development Program of Guangdong Province [2020B1111110006]
- Hong Kong RGC-GFC [16100921]
- Zhongshan Municipal Bureau of Sci-ence and Technology [2019AG035]
- Guangzhou Science and Technology Committee Research Grant [GZSTI16SC02, GZSTI17SC02]
- GBA Institute of Collaborate Innovation [GICI-022]
- Special Project of Foshan University of Science and Technology in 2019 [FSUST19-SRI10]
- Hong Kong RGC Theme-based Research Scheme [T13-605/18-W]
- Hong Kong Innovation Technology Fund [PRP/076/20FX, UIM/385, ITS/500/18FP, ITCPD/17-9]
- Shenzhen Science and Technology Innovation Committee [ZDSYS201707281432317]
- [TUYF19SC02]
- [PD18SC01]
- [HMRF18SC06]
- [HMRF20SC07]
- [AFD20SC01]
In this study, a multi-component testing platform was used to identify potential therapeutic agents for COVID-19 among herbal extracts from traditional Chinese medicine. The water and ethanol extracts of Polygoni Multiflori Radix were found to effectively inhibit the binding and entry of SARS-CoV-2, with the water extract being more effective. The active component EGCG was identified in both extracts. These findings suggest that Polygoni Multiflori Radix may have potential as a treatment for COVID-19.
Background: Globally, COVID-19 has caused millions of deaths and led to unprecedented socioeconomic damage. There is therefore, in addition to vaccination, an urgent need to develop complementary effective treatments and/or protective and preventative therapies against this deadly disease. Methods: Here, a multi-component testing platform was established to screen a library of herbal extracts from traditional Chinese medicine (TCM), to identify potent herbal extracts/phytochemicals as possible therapeutics for COVID-19. We utilized assays for spike protein (S-protein) binding to angiotensin-converting enzyme II (ACE2); the enzymatic inhibition of 3CL protease; and entry of the SARS-CoV-2 pseudovirus into cultured HEK293T cells and zebrafish larvae. Results: Over a thousand herbal extracts were screened and approximately 20 positive hits were identified. Among these, we found that the water and ethanol extracts of Polygoni Multiflori Radix (PMR) significantly inhibited S-protein binding to ACE2, 3CL protease activity, and viral entry into the cell and fish models. The water extract was more effective than the ethanol extract, with IC50 values of 25 to 500 mu g/ml. In addition, the polysaccharide-depleted fraction of the former, and epigallocatechin gallate (EGCG) which was found in both extracts, displayed significant antiviral activity. Conclusions: Our results indicate that the water and ethanol extracts of PMR have an inhibitory effect on SARS-CoV-2 pseudovirus host-cell entry. Furthermore, EGCG might be an active component of PMR, which blocks SARS-CoV-2 entry to cells. Taken together, our findings suggest that PMR might be considered as a potential treatment for COVID-19.
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