期刊
PHYTOCHEMISTRY
卷 203, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2022.113355
关键词
Ginkgo biloba; Ginkgoaceae; Ginkgolide; Characteristic ion; Neutral loss; Antiplatelet aggregation activities
资金
- Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences [CI2021A4404, CI2021A04405]
- Major Scientific and Technological Special Project for the Fundamental Research Funds for the Central public welfare research institutes [ZZ13-YQ-057, ZZ13-YQ-061, ZXKT21006]
- National Key Research and Development Project [2019YFC19066-01]
In this study, a global profile of characteristic ion and neutral loss recognition strategy was used to discover eight undescribed ginkgolides and eight known ginkgolides in Ginkgo biloba L. These compounds exhibited antiplatelet aggregation activities and enriched the known structural diversity of ginkgolides. The application of mass spectrometry in the global profiling of natural products present in Ginkgo biloba L. was extended, which could aid chemists in rapidly discovering unreported compounds from a complex matrix.
Ginkgolides are the most important bioactive components of Ginkgo biloba L, of which ginkgolide B has been successfully developed and marketed as a drug. The reported ginkgolides are very rare and exhibit a complex matrix due to the chemodiversity of Ginkgo biloba L. Herein, the global profile of characteristic ion and neutral loss recognition strategy were used for to discover eight undescribed ginkgolides, very rare cyclohexane ginkgolides R-V, ginkgolides D-F, and eight known ginkgolides. These ginkgolides were target isolated and identified using high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy, and X-ray crystallography. The undescribed and known ginkgolides exhibited antiplatelet aggregation activities. In particular, compounds U and D had IC50 values of 2.20 +/- 0.15 and 6.50 +/- 0.87 mu M, respectively. This study has enriched the known structural diversity of ginkgolides and extended the application of mass spectrometry to the global profiling of natural products present in Ginkgo biloba L. Moreover, it could help chemists rapidly discover unreported compounds from a complex matrix.
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