期刊
OPTICS EXPRESS
卷 30, 期 15, 页码 26396-26406出版社
Optica Publishing Group
DOI: 10.1364/OE.456871
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资金
- National Institute of Biomedical Imaging and Bioengineering [R01EB01548]
- National Science Foundation [2016035]
- GG Gift Fund
- Direct For Biological Sciences
- Div Of Biological Infrastructure [2016035] Funding Source: National Science Foundation
Two-photon light-sheet fluorescence microscopy allows high-resolution imaging of neural activity in brain tissue at a high frame rate. The traditional light-sheet microscopy technique has limitations in volumetric imaging speed, but with the introduction of depth random-access light-sheet microscopy, rapid switching of scanning depth for light-sheet imaging is possible, resulting in faster imaging and higher refreshing rates.
Two-photon light-sheet fluorescence microscopy enables high-resolution imaging of neural activity in brain tissue at a high frame rate. Traditionally, light-sheet microscopy builds up a 3D stack by multiple depth scans with uniform spatial intervals, which substantially limits the volumetric imaging speed. Here, we introduce the depth random-access light-sheet microscopy, allowing rapid switching scanning depth for light-sheet imaging. With a low-cost electrically tunable lens and minimum modification of an existing two-photon light-sheet imaging instrument, we demonstrated fast random depth hopping light-sheet imaging at 100 frames per second in the live brain slice. Through depth random-access, calcium activities for an astrocyte were recorded on four user-selected detection planes at a refreshing rate of 25 Hz. (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
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