期刊
NUCLEIC ACIDS RESEARCH
卷 50, 期 14, 页码 7842-7855出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac582
关键词
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资金
- Cancer Prevention & Research Institute of Texas (CPRIT) Award [RR170030]
- National Institutes of Health R35 Award [R35GM143532]
- NIH
This study systematically compared different dCas9-based transcriptional activators and found that their expression levels and transactivation efficacies vary in different human cell types. The study also showed that these activators can induce the production of enhancer RNAs, which are positively correlated with downstream mRNA expression. Additionally, the study demonstrated the existence of intrinsic transcriptional and epigenetic reciprocity between human enhancers and promoters.
Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at human enhancer-promoter pairs. Here, we systematically compared the most widely adopted dCas9-based transcriptional activators, as well as an activator consisting of dCas9 fused to the catalytic core of the human CBP protein, at human enhancer-promoter pairs. We find that these platforms display variable relative expression levels in different human cell types and that their transactivation efficacies vary based upon the effector domain, effector recruitment architecture, targeted locus and cell type. We also show that each dCas9-based activator can induce the production of enhancer RNAs (eRNAs) and that this eRNA induction is positively correlated with downstream mRNA expression from a cognate promoter. Additionally, we use dCas9-based activators to demonstrate that an intrinsic transcriptional and epigenetic reciprocity can exist between human enhancers and promoters and that enhancer-mediated tracking and engagement of a downstream promoter can be synthetically driven by targeting dCas9-based transcriptional activators to an enhancer. Collectively, our study provides new insights into the enhancer-mediated control of human gene expression and the use of dCas9-based activators.
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