期刊
NEW PHYTOLOGIST
卷 236, 期 2, 页码 447-463出版社
WILEY
DOI: 10.1111/nph.18348
关键词
abscisic acid; AGB1; Arabidopsis; G-protein; photosynthesis; proteomics; redox; redox proteomics
资金
- National Science Foundation [IOS-1557942, MCB-1714693]
- National Science Foundation CAREER award [MCB-1552522]
This study investigates the role of reversible protein oxidation in plant stress response and identifies a complex network of reversible oxidations associated with ABA and G-protein signaling. The findings indicate that functional G-proteins are required to maintain intracellular redox homeostasis and fully execute plant stress responses.
The plant hormone abscisic acid (ABA) plays crucial roles in regulation of stress responses and growth modulation. Heterotrimeric G-proteins are key mediators of ABA responses. Both ABA and G-proteins have also been implicated in intracellular redox regulation; however, the extent to which reversible protein oxidation manipulates ABA and/or G-protein signaling remains uncharacterized. To probe the role of reversible protein oxidation in plant stress response and its dependence on G-proteins, we determined the ABA-dependent reversible redoxome of wild-type and G beta-protein null mutant agb1 of Arabidopsis. We quantified 6891 uniquely oxidized cysteine-containing peptides, 923 of which show significant changes in oxidation following ABA treatment. The majority of these changes required the presence of G-proteins. Divergent pathways including primary metabolism, reactive oxygen species response, translation and photosynthesis exhibited both ABA- and G-protein-dependent redox changes, many of which occurred on proteins not previously linked to them. We report the most comprehensive ABA-dependent plant redoxome and uncover a complex network of reversible oxidations that allow ABA and G-proteins to rapidly adjust cellular signaling to adapt to changing environments. Physiological validation of a subset of these observations suggests that functional G-proteins are required to maintain intracellular redox homeostasis and fully execute plant stress responses.
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