4.4 Article

Systemic administration of lipopolysaccharide induces hyperexcitability of prelimbic neurons via modulation of sodium and potassium currents

期刊

NEUROTOXICOLOGY
卷 91, 期 -, 页码 128-139

出版社

ELSEVIER
DOI: 10.1016/j.neuro.2022.05.010

关键词

LPS; Prelimbic cortex; Sodium channels; Potassium channels; Intrinsic excitability

资金

  1. Consejo Nacional de Ciencia y Tecnologia, Mexico [CB-2016-281617]
  2. [718589]
  3. [727269]

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This study demonstrated that immune activation alters the ionic currents shaping the intrinsic excitability of prefrontal cortex neurons, predicting dysregulation of non-synaptic forms of neuronal plasticity modulated by intrinsic excitability.
In C57BL/6 J mice, systemic inflammation was induced by administering bacterial LPS (1 mg/kg) intraperitoneally. In response, animals exhibited hypokinesia, piloerection, and a slight decrease in body temperature accompanied by increased serum levels of the proinflammatory cytokine TNF-alpha. 24 h after the immunogenic challenge, acute cortical slices were prepared, and whole-cell patch-clamp recordings were performed in morphologically identified prelimbic neurons of the mice's prefrontal cortex. Electrophysiologic alterations included changes in the kinetics parameters of the fast-inactivating sodium and slow-inactivating potassium currents. In current-clamp mode, our recordings revealed alterations in several conductances that shape the intrinsic excitability of prelimbic neurons. The action potential exhibited changes in latency, amplitude, and the rheobase current to elicit firing discharge. Likewise, phase plots of the action potentials uncovered alterations in the repetitive firing of prelimbic neurons. Consistent with these changes, the afterhyperpolarization conductance and the slowly decaying, calcium-dependent after-hyperpolarization current that follows an action potential were decreased in response to systemic LPS. Our data demonstrate that immune activation alters the ionic currents that shape the intrinsic excitability and predicts dysregulation of non-synaptic forms of neuronal plasticity modulated by the intrinsic excitability of prefrontal cortex neurons.

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