4.4 Article

Intestinal dysbacteriosis-propelled T helper 17 cells activation mediate the perioperative neurocognitive disorder induced by anesthesia/surgery in aged rats

期刊

NEUROSCIENCE LETTERS
卷 783, 期 -, 页码 -

出版社

ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2022.136741

关键词

Th17; IL17; Microglia; PND; Intestinal flora

资金

  1. National Natural Science Foundation of China [82071196, 81801074]
  2. Jiangsu Commission of Health [z201949]
  3. Science and Technology Development Fund of Nanjing Medical University [NMUB20210128]
  4. Basic Research Grant of Southeast University

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The study found that surgery/anesthesia caused an imbalance in intestinal flora, leading to neurocognitive impairment, increased the number of Th17 cells in various organs, and elevated levels of IL17, IL17R, and inflammatory factors in the hippocampus. Administration of antibiotics before surgery/anesthesia significantly reduced Th17 cell numbers, IL17 levels, IL17R levels, and inflammatory factor production, thereby improving memory function.
Background: Perioperative neurocognitive disorders (PND) is a common postoperative disease in elderly patients, but its pathogenesis remains unclear.Methods: Exploratory laparotomy was performed to establish PND model under sevoflurane anesthesia. 16S rRNA high-throughput sequencing was used to detect the changes of intestinal flora. Antibiotics were used to relatively eliminate intestinal flora before anesthesia/surgery, and behavior tests, such as open field, Y maze, and fear conditioning tests were applied to detect the changes of memory ability. The number of Th17 cells and Foxp3 cells was detected by flow cytometry in the Peyer's patches (PP), mesenteric lymph nodes (MLN), blood and brain. Western blot was used to detect the expression of IL17, IL17RA, IL6 and IL10 in the hippocampus. Immunofluorescence was used to detect the expression of IL17, IL17R and IBA1 (ionized calcium binding adaptor molecule1) in the hippocampus.Results: Anesthesia/surgery caused intestinal flora imbalance and induced neurocognitive impairment, increased the number of Th17 cells in the PP, MLN, blood and brain, increased the level of IL17, IL17R and inflammatory factors production in the hippocampus. Antibiotics administration before anesthesia/surgery significantly decreased the number of Th17 cells and the level of IL17, IL17R and inflammatory factors production, and improved the memory function. In addition, we found that IL17R was co-labeled with IBA1 in a large amount in the hippocampus through immunofluorescence double-staining.Conclusion: Our study suggested that intestinal dysbacteriosis-propelled T helper 17 cells activation and IL17 secretion might play an important role in the pathogenesis of PND induced by anesthesia/surgery in aged rats.

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