期刊
NEURO-ONCOLOGY
卷 25, 期 1, 页码 82-96出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/noac157
关键词
glioma stem cells (GSCs); glioblastoma multiforme; Oct4A; palmitoylation; Sox2
In this study, it was found that Oct4A can impact the tumorigenic activity of glioblastoma and its stability is maintained through palmitoylation. Oct4A palmitoylation helps to integrate Sox4 and Oct4A in the SOX2 enhancement subregion, thereby maintaining the stem performance of glioma stem cells. The study also designed Oct4A palmitoylation competitive inhibitors that can inhibit the self-renewal ability and tumorigenicity of glioma stem cells.
Background Glioblastoma multiforme and other solid malignancies are heterogeneous, containing subpopulations of tumor cells that exhibit stem characteristics. Oct4, also known as POU5F1, is a key transcription factor in the self-renewal, proliferation, and differentiation of stem cells. Although it has been detected in advanced gliomas, the biological function of Oct4, and transcriptional machinery maintained by the stemness of Oct4 protein-mediated glioma stem cells (GSC), has not been fully determined. Methods The expression of Oct4 variants was evaluated in brain cancer cell lines, and in brain tumor tissues, by quantitative real-time PCR, western blotting, and immunohistochemical analysis. The palmitoylation level of Oct4A was determined by the acyl-biotin exchange method, and the effects of palmitoylation Oct4A on GSCs were investigated by a series of in vitro (neuro-sphere formation assay, double immunofluorescence, pharmacological treatment, luciferase assay, and coimmunoprecipitation) and in vivo (xenograft model) experiments. Results Here, we report that all three variants of Oct4 are expressed in different types of cerebral cancer, while Oct4A is important for maintaining tumorigenicity in GSCs. Palmitoylation mediated by ZDHHC17 was indispensable for preserving Oct4A from lysosome degradation to maintain its protein stability. Oct4A palmitoylation also helped to integrate Sox4 and Oct4A in the SOX2 enhancement subregion to maintain the stem performance of GSCs. We also designed Oct4A palmitoylation competitive inhibitors, inhibiting the self-renewal ability and tumorigenicity of GSCs. Conclusions These findings indicate that Oct4A acts on the tumorigenic activity of glioblastoma, and Oct4A palmitoylation is a candidate therapeutic target.
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