期刊
NATURE METHODS
卷 19, 期 7, 页码 823-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-022-01539-7
关键词
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资金
- VILLUM FONDEN [15510]
- Poul Due Jensen Foundation (Microflora Danica)
This study demonstrates that near-finished microbial genomes can be generated using only Oxford Nanopore R10.4 data, without the need for short-read or reference polishing. This finding simplifies the microbial genome sequencing process and improves the accuracy and continuity of sequencing results.
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing. This study demonstrates the feasibility of generating near-finished microbial genomes using only Oxford Nanopore R10.4 data from pure cultures or metagenomes.
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