期刊
NATURE
卷 607, 期 7919, 页码 527-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-022-04912-w
关键词
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资金
- National Institutes of Health [K01MH125144, R35NS097370, R35NS116843]
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- Lieber Institute for Brain Development
This study identified molecular characteristics of immature dentate granule cells (imGCs) in the adult human hippocampus using single-nucleus RNA sequencing, revealing common molecular hallmarks across the lifespan and age-dependent transcriptional dynamics. The study also showed a decreased number of imGCs in Alzheimer's disease and demonstrated neurogenesis in the adult human hippocampus.
Immature dentate granule cells (imGCs) arising from adult hippocampal neurogenesis contribute to plasticity and unique brain functions in rodents(1,2) and are dysregulated in multiple human neurological disorders(3-5). Little is known about the molecular characteristics of adult human hippocampal imGCs, and even their existence is under debate(1,6-8). Here we performed single-nucleus RNA sequencing aided by a validated machine learning-based analytic approach to identify imGCs and quantify their abundance in the human hippocampus at different stages across the lifespan. We identified common molecular hallmarks of human imGCs across the lifespan and observed age-dependent transcriptional dynamics in human imGCs that suggest changes in cellular functionality, niche interactions and disease relevance, that differ from those in mice(9). We also found a decreased number of imGCs with altered gene expression in Alzheimer's disease. Finally, we demonstrated the capacity for neurogenesis in the adult human hippocampus with the presence of rare dentate granule cell fate-specific proliferating neural progenitors and with cultured surgical specimens. Together, our findings suggest the presence of a substantial number of imGCs in the adult human hippocampus via low-frequency de novo generation and protracted maturation, and our study reveals their molecular properties across the lifespan and in Alzheimer's disease.
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