期刊
NATURE
卷 609, 期 7925, 页码 128-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-022-05074-5
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资金
- Howard Hughes Medical Institute (HHMI)
- National Institute of Health (NIH) [R01-NS097161]
- Agence Nationale de la Recherche [ANR-20-CE92-0053]
- Damon Runyon Postdoctoral Fellowship
- Agence Nationale de la Recherche (ANR) [ANR-20-CE92-0053] Funding Source: Agence Nationale de la Recherche (ANR)
This study reveals an important mechanism for maintaining neuronal polarity in the axon initial segment (AIS), which involves the endocytic removal of polarized receptors that diffuse into the AIS. This mechanism works together with the known AIS diffusion-barrier mechanism to reinforce the membrane boundaries between axonal and dendritic compartments.
Neurons are highly polarized cells that face the fundamental challenge of compartmentalizing a vast and diverse repertoire of proteins in order to function properly(1). The axon initial segment (AIS) is a specialized domain that separates a neuron's morphologically, biochemically and functionally distinct axon and dendrite compartments(2,3). How the AIS maintains polarity between these compartments is not fully understood. Here we find that in Caenorhabditis elegans, mouse, rat and human neurons, dendritically and axonally polarized transmembrane proteins are recognized by endocytic machinery in the AIS, robustly endocytosed and targeted to late endosomes for degradation. Forcing receptor interaction with the AIS master organizer, ankyrinG, antagonizes receptor endocytosis in the AIS, causes receptor accumulation in the AIS, and leads to polarity deficits with subsequent morphological and behavioural defects. Therefore, endocytic removal of polarized receptors that diffuse into the AIS serves as a membrane-clearance mechanism that is likely to work in conjunction with the known AIS diffusion-barrier mechanism to maintain neuronal polarity on the plasma membrane. Our results reveal a conserved endocytic clearance mechanism in the AIS to maintain neuronal polarity by reinforcing axonal and dendritic compartment membrane boundaries.
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